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5 March 2021 Multicolor two-photon microscopy for living mice using broadband fiber-continuum selective excitation and linear unmixing
Xinyuan Huang, Zhongyun Chen, Ling Fu
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Proceedings Volume 11641, Dynamics and Fluctuations in Biomedical Photonics XVIII; 1164107 (2021) https://doi.org/10.1117/12.2578385
Event: SPIE BiOS, 2021, Online Only
Abstract
Here we proposed an imaging strategy to monitor more fluorescent objectives synchronously in the living animals with multicolor two-photon excited fluorescence microscopy. We used highly nonlinear photonic crystal fibers and a 100-fs Ti: Sapphire oscillator to generate 200 nm wide continuum pulses, which covers the two-photon excitation spectra of conventional fluorophores. Then, we used the phase shaping method to compensate for the dispersion and switch excitation wavelength rapidly. Next, we implemented non-negative matrix factorization (NMF) to unmix images with cross-talk. Images of 8-color HeLa cells and 4-color mice tumor under the mouse dorsal skinfold chamber were acquired.
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Xinyuan Huang, Zhongyun Chen, and Ling Fu "Multicolor two-photon microscopy for living mice using broadband fiber-continuum selective excitation and linear unmixing", Proc. SPIE 11641, Dynamics and Fluctuations in Biomedical Photonics XVIII, 1164107 (5 March 2021); https://doi.org/10.1117/12.2578385
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KEYWORDS
Two photon excitation microscopy
Imaging systems
Microscopy
Tissue optics
Tumors
Optical damage
Oscillators
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