Quantitative multiplexing in biological tissue using multispectral and fluorescence lifetime imaging

Rahul Pal; Anand T. N. Kumar

doi:10.1117/12.2578437

5 March 2021 Quantitative multiplexing in biological tissue using multispectral and fluorescence lifetime imaging

Rahul Pal, Anand T. N. Kumar

Proceedings Volume 11639, Optical Tomography and Spectroscopy of Tissue XIV; 116391V (2021) https://doi.org/10.1117/12.2578437
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

The well-known red shift of fluorescence spectra in thick tissue causes significant cross-talk and inaccurate quantification of fluorophores using multispectral imaging (MSI). However, fluorescence lifetimes (FLT) are largely unaffected by tissue light propagation and results in superior quantitation accuracy. Here we present a systematic experimental comparison of quantitative MSI and FLT of multiple fluorescent molecules in tissue. In experiments using one or two fluorescence species in mixture with tissue autofluorescence, MSI showed significantly higher error rates (>20%) than FLT (<5%). These results show promise for in vivo FLT multiplexing based imaging of receptor expression in cancer and other diseases.

Conference Presentation

Citation Download Citation

Rahul Pal and Anand T. N. Kumar "Quantitative multiplexing in biological tissue using multispectral and fluorescence lifetime imaging", Proc. SPIE 11639, Optical Tomography and Spectroscopy of Tissue XIV, 116391V (5 March 2021); https://doi.org/10.1117/12.2578437

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