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We propose a novel Time-Resolved Mesoscopic Fluorescence Molecular Tomography (TR-MFMT) system based around a supercontinuum laser across visible to NIR range and a customized Single-Photon Avalanche Diode (SPAD) array. The system characterization is performed within the settings of multi-spectral fluorescence imaging. Especially, the characterization is achieved with a challenging application: NIR FRET quantification. The characterization results demonstrate the utility of narrow instrument response function systems and provide the foundation for high-resolution imaging multiplexed molecular imaging in the mesoscopic regime.
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