Phase-contrast optical microscopy using active image processing

Masaki Hisaka

doi:10.1117/12.2573220

15 June 2020 Phase-contrast optical microscopy using active image processing

Masaki Hisaka

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 115210A (2020) https://doi.org/10.1117/12.2573220
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

Phase-contrast imaging of biological tissues is one of the most important techniques used to investigate biological structures and functions. A phase-contrast optical microscope using oblique illumination and active image processing that is a figure-8-shaped spatial-frequency filter with sign changes has been developed to visualize phase structure of living tissues. This microscope offers promising features including separate phase and amplitude-contrast imaging, spherical aberration-free imaging, and reduced multi-scattering light. We reproduced and evaluated phase-contrast images of biological samples. We also extended the technique to the phase-contrast scanning microscope using annular illumination.

Citation Download Citation

Masaki Hisaka "Phase-contrast optical microscopy using active image processing", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 115210A (15 June 2020); https://doi.org/10.1117/12.2573220

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