Metabolic shifts in liver diseases and regeneration (FLIM and TOF SIMS) (Conference Presentation)

Elena V. Zagaynova; Svetlana A. Rodimova; Daria Kuznetsova; Dmitry Reunov; Alexander Gulin; Nikolay Bobrov; Natalia Vdovina; Vladimir E. Zagainov

doi:10.1117/12.2555655

8 April 2020 Metabolic shifts in liver diseases and regeneration (FLIM and TOF SIMS) (Conference Presentation)

Elena V. Zagaynova, Svetlana A. Rodimova, Daria Kuznetsova, Dmitry Reunov, Alexander Gulin, Nikolay Bobrov, Natalia Vdovina, Vladimir E. Zagainov

Author Affiliations +

Proceedings Volume 11363, Tissue Optics and Photonics; 113630K (2020) https://doi.org/10.1117/12.2555655
Event: SPIE Photonics Europe, 2020, Online Only

Abstract

Conventional techniques are insufficient to precisely describe the internal structure, heterogeneous cell populations and the dynamics of biological processes of the diseased liver during and after surgery. There is a need for a rapid and safe method for the successful diagnosis of liver disease to plan surgery, efficacy of regeneration and avoid postoperative liver failure. Here, we analyze acute and chronic liver pathology (cholestasis, cirrhosis, fatty liver disease) during progression by multiphoton microscopy with FLIM and SHG modes and chemical analysis by TOF-SIMS to obtain new data about hepatocyte pathological changes at the cellular and molecular level. All of these techniques enable to study cellular metabolism, lipid composition and collagen structure without sample staining and incorporation of fluorescent or another markers that may allow to use these methods in clinic. Complex of multiphoton microscopy and mass spectrometry provides complete information about the liver structure and function that could not be assessed using each method individually. The data can be used for both to obtain new criteria for the identification of hepatic pathology and to develop a rapid technique of liver quality analysis to plan surgery and avoid postoperative liver failure in clinic.

Conference Presentation

Citation Download Citation

Elena V. Zagaynova, Svetlana A. Rodimova, Daria Kuznetsova, Dmitry Reunov, Alexander Gulin, Nikolay Bobrov, Natalia Vdovina, and Vladimir E. Zagainov "Metabolic shifts in liver diseases and regeneration (FLIM and TOF SIMS) (Conference Presentation)", Proc. SPIE 11363, Tissue Optics and Photonics, 113630K (8 April 2020); https://doi.org/10.1117/12.2555655

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available