An orange-fluorescent dye with long-lasting blinking for single molecule localization microscopy

Daiki Hara; Yuji Okamoto; Shingo Fujiyama; Youichi Nishikawa; Yoshihiko Norimine; Yuji Kazuta; Takafumi Motoki; Hiroyuki Amino; Shigeki Iwanaga

doi:10.1117/12.2544492

21 February 2020 An orange-fluorescent dye with long-lasting blinking for single molecule localization microscopy

Daiki Hara, Yuji Okamoto, Shingo Fujiyama, Youichi Nishikawa, Yoshihiko Norimine, Yuji Kazuta, Takafumi Motoki, Hiroyuki Amino, Shigeki Iwanaga

Author Affiliations +

Proceedings Volume 11256, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XII; 112560G (2020) https://doi.org/10.1117/12.2544492
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Single molecule localization microscopy (SMLM) relies on the detection of fluorescence emission from a single fluorophore molecule. Recently, several fluorescent dyes with spontaneous blinking have been reported. Last year, we reported a near-infrared dye with spontaneous blinking and high photostability. Here, we present a new orangefluorescent dye that exhibits spontaneous blinking based on equilibrium between the fluorescent and non-fluorescent forms. We have also ascertained that the new dye can be used to create a super-resolution image of cytoskeletal microtubules. In combination with our near-infrared dye reported last year, the new dye is expected to allow dual-color SMLM.

Citation Download Citation

Daiki Hara, Yuji Okamoto, Shingo Fujiyama, Youichi Nishikawa, Yoshihiko Norimine, Yuji Kazuta, Takafumi Motoki, Hiroyuki Amino, and Shigeki Iwanaga "An orange-fluorescent dye with long-lasting blinking for single molecule localization microscopy", Proc. SPIE 11256, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XII, 112560G (21 February 2020); https://doi.org/10.1117/12.2544492

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