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The invention of Multiphoton excitation microscopy took place 30 years ago. The tremendous success and proliferation of applications related to this non-linear imaging modality resulted in the development of femtosecond tunable lasers specifically designed to address varieties of probes and optical set-ups. Recent innovations like three-photon imaging, optogenetics activation of many neurons and new approaches towards diagnostic applications fostered an even more rapid pace of innovation resulting in laser sources producing high energy tunable pulses and compact, dedicated and cost-effective sources. We will describe these new sources and how they fit diverse applications within Multiphoton excitation Microscopy
Marco Arrigoni andDarryl McCoy
"New paradigms in femtosecond lasers for non-linear imaging of the brain and other tissues (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112440G (9 March 2020); https://doi.org/10.1117/12.2548655
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Marco Arrigoni, Darryl McCoy, "New paradigms in femtosecond lasers for non-linear imaging of the brain and other tissues (Conference Presentation)," Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112440G (9 March 2020); https://doi.org/10.1117/12.2548655