CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 11190 > Article
Paper
20 November 2019 Fan-shaped tracker (FsT) for particle trajectory reconstruction
Luhong Jin, Fenqiang Zhao, Wanni Lin, Xiaoxu Zhou, Cuifang Kuang, Liu Xu, Sergey Ablameyko, Yingke Xu
Author Affiliations +
Proceedings Volume 11190, Optics in Health Care and Biomedical Optics IX; 111900Y (2019) https://doi.org/10.1117/12.2538450
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China
Abstract
With the development of super-resolution fluorescence microscopy, complex dynamic processes in living cells can be observed and recorded with unprecedented temporal and spatial resolution. Single particle tracking is the most important step to explore the relationship between the spatio-temporal dynamics of subcellular molecules and their functions. Although previous studies have developed single particle tracking algorithms to quantitatively analyze particle dynamics in cell, traditional tracking methods have poor performance when dealing with intersecting trajectories. This can be attributed to two main reasons: 1) They do not have point compensation process for overlapping points; 2) They use inefficient motion prediction models. In this paper, we presented a novel Fan-shaped Tracker (FsT) algorithm to reconstruct the trajectories of subcellular molecules in living cells. We proposed a customized point compensation method for overlapping points based on the fan-shape motion trend of the particles to solve the merging trajectory problem. Furthermore, we compared the performance of our Fan-shaped Tracker with five state-of-the-art tracking algorithms in simulated time-lapse movies with variable imaging quality. Our results showed that the Fan-shaped Tracker achieves better performance than other reported methods as we systematically evaluated using a set of standard evaluation parameters. We anticipate that our FsT method will have vast applications in tracking of moving objects in cell.
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Luhong Jin, Fenqiang Zhao, Wanni Lin, Xiaoxu Zhou, Cuifang Kuang, Liu Xu, Sergey Ablameyko, and Yingke Xu "Fan-shaped tracker (FsT) for particle trajectory reconstruction", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111900Y (20 November 2019); https://doi.org/10.1117/12.2538450
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 9 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Particles
Signal to noise ratio
Detection and tracking algorithms
Image processing
Algorithm development
Microscopy
Motion models
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top