Frequency shifting confocal microscopy via azimuthally polarized Bessel-Gaussian beam

Yong Liu; Cuifang Kuang

doi:10.1117/12.2537729

19 November 2019 Frequency shifting confocal microscopy via azimuthally polarized Bessel-Gaussian beam

Yong Liu, Cuifang Kuang

Proceedings Volume 11186, Advanced Optical Imaging Technologies II; 111861B (2019) https://doi.org/10.1117/12.2537729
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

A new frequency-shifting confocal microscopy (FSCM) illuminated by an azimuthally polarized Bessel-Gaussian (BG) beam is investigated. The solid excitation spot is produced by the BG beam modulated by a spiral phase plate, and the donut excitation spot is directly obtained by the same BG beam. Through vector diffraction theory and two-view RL reconstruction algorithm, the optical transfer functions of two confocal imaging modes and the simulation imaging of FSCM are presented. The results show that, two illumination modes produced by the azimuthally polarized BG beam can enhance the spatial resolution of FSCM, the spatial resolution of reconstructed image is mainly depended on the illumination mode with higher frequency transfer efficiency, the small pinhole is helpful to improve the contrast and spatial resolution of image. When the iterations number is about 100, the reconstructed image has good quality. This FSCM is helpful to quickly realize super resolution and high contrast in cell imaging.

Citation Download Citation

Yong Liu and Cuifang Kuang "Frequency shifting confocal microscopy via azimuthally polarized Bessel-Gaussian beam", Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 111861B (19 November 2019); https://doi.org/10.1117/12.2537729

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Confocal microscopy

Spatial frequencies

Optical transfer functions

Spatial resolution

Luminescence

Image processing

Modulation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years