Optoelectronical methods for screening of Pralmorelin and its metabolite in urine samples

Jeni-Carla Colev; Bogdan Berghes; Mihai Radu; Mihai Ionică; Georgeta Marcela Bican

doi:10.1117/12.2323812

31 December 2018 Optoelectronical methods for screening of Pralmorelin and its metabolite in urine samples

Jeni-Carla Colev, Bogdan Berghes, Mihai Radu, Mihai Ionică, Georgeta Marcela Bican

Proceedings Volume 10977, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies IX; 109773W (2018) https://doi.org/10.1117/12.2323812
Event: Advanced Topics in Optoelectronics, Microelectronics and Nanotechnologies IX, 2018, Constanta, Romania

Abstract

GHRP-2, also known as Pralmorelin, is a synthetic peptide drug. It acts as ghrelin/growth-hormone secretagogue receptor agonist, being the first drug of this class introduced clinically. It's used, in a single dose formulation, as a diagnostic agent for growth hormone deficiency (GHD). Administration of GHRP-2 increases the plasmatic concentration of growth hormone. It was clinically tested for tratment of GHD and pituitary dwarfism. GHRP-2 has performance enhancing potential and is prohibited for athletes in section S2: Peptide Hormones, Growth Factors, Related Substances, And Mimetics of WADA (World Antidoping Agency) Prohibited List. In this work we developed and validated a screening method for GHRP-2 and it's two metabolites from urine on LC-HRMS (liquid chromatography coupled with high resolution mass spectrometry)(Q Exactive Plus from Thermo Scientific) and LC-MS/MS triple quadrupole (liquid chromatography coupled with mass spectrometry) (ABSciex QTrap 5500). The validation parameters evaluated were limit of detection, matrix effects, identification criteria, specificity, carry-over and extraction recovery. the evaluated parameters are in accordance with WADA technical documents, both methods being applicable for doping control application.

Citation Download Citation

Jeni-Carla Colev, Bogdan Berghes, Mihai Radu, Mihai Ionică, and Georgeta Marcela Bican "Optoelectronical methods for screening of Pralmorelin and its metabolite in urine samples", Proc. SPIE 10977, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies IX, 109773W (31 December 2018); https://doi.org/10.1117/12.2323812

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