Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser

Lin Huang; Shuo Tang

doi:10.1117/12.2505845

22 February 2019 Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser

Lin Huang, Shuo Tang

Author Affiliations +

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108822G (2019) https://doi.org/10.1117/12.2505845
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

A multimodal multiphoton microscopy (MPM) is developed to acquire two-photon and three-photon signals simultaneously, including two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and third harmonic generation (THG). We have developed a miniature multimodal MPM system based on a dual-wavelength Erdoped fiber laser, which includes the fundamental pulse at 1580 nm to excite THG and the frequency-doubled pulse at 790 nm to excite TPEF and SHG. The laser is coupled by a single mode fiber into a miniature MPM imaging probe. Label-free imaging by TPEF, SHG, and THG are demonstrated on biological samples, obtained from intrinsic fluorophores, collagen, lipid and interface.

Citation Download Citation

Lin Huang and Shuo Tang "Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108822G (22 February 2019); https://doi.org/10.1117/12.2505845

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available