Ophthalmic FLIM for eye disease studies: advanced technical solutions  (Conference Presentation)

Cornelia Junghans; Hauke Studier; Wolfgang Becker

doi:10.1117/12.2509872

4 March 2019 Ophthalmic FLIM for eye disease studies: advanced technical solutions (Conference Presentation)

Cornelia Junghans, Hauke Studier, Wolfgang Becker

Author Affiliations +

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108820X (2019) https://doi.org/10.1117/12.2509872
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Ophthalmic imaging by fluorescence techniques is a tool which gets more and more established in eye disease diagnosis and research. All type of clinical imaging is usually restricted to the use of endogenous fluorophores present in the tissue. The excitation and emission spectra of these fluorophores are overlapping and poorly defined. Moreover, the apparent spectra are changed by variation in the relative concentration of fluorophores and by absorbers present in the tissue. Intensity images, even those with spectral resolution, therefore deliver very limited information on the state of the tissue. A considerable improvement in the field of retinal imaging is obtained by using fluorescence lifetime imaging ophthalmoscopy (FLIO). The fluorescence lifetime measured by TCSPC is independent of the concentration, and enables the possibility to measure even the weak retinal autofluorescence. Moreover, it delivers direct information on the configuration of endogenous fluorophores, on binding to proteins or lipids, on the redox state, and on other metabolic parameters. We will describe the technical problems of FLIO data and their solutions, demonstrate the performance of existing systems in ophthalmology and present some results.

Conference Presentation

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Cornelia Junghans, Hauke Studier, and Wolfgang Becker "Ophthalmic FLIM for eye disease studies: advanced technical solutions (Conference Presentation)", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108820X (4 March 2019); https://doi.org/10.1117/12.2509872

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KEYWORDS

Eye

Fluorescence lifetime imaging

Tissues

Luminescence

Ophthalmic imaging

Ophthalmology

Ophthalmoscopy

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