Multiphoton imaging and OCT MA for diagnosis of human melanocytic lesions

Elena V. Zagaynova; Vadim Elagin; Ekatarina Gubarkova; Oksana Garanina; Natalia Orlinskaya; Varvara V. Dudenkova; Irena Shlivko; Irina Klemenova; Diana Davydova

doi:10.1117/12.2508480

22 February 2019 Multiphoton imaging and OCT MA for diagnosis of human melanocytic lesions

Elena V. Zagaynova, Vadim Elagin, Ekatarina Gubarkova, Oksana Garanina, Natalia Orlinskaya, Varvara V. Dudenkova, Irena Shlivko, Irina Klemenova, Diana Davydova

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Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108820G (2019) https://doi.org/10.1117/12.2508480
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

The clinical diagnosis of melanocytic lesions is an ongoing medical challenge. Non-invasive tools and technologies can help to distinguish equivocal lesions. The aim of the study was to improve the in vivo diagnostic possibilities for the differentiation of benign and malignant melanocytic lesions based on combination of three imaging label-free modalities (multiphoton tomography, fluorescence lifetime imaging and optical coherence angiography). Thirty-two melanocytic lesions were studied, using multiphoton tomography, fluorescence lifetime imaging and optical coherence angiography. Multiphoton tomography features of benign melanocytic nevi were characterized by the normal morphology of both the keratinocytes and the nevus cell nests surrounded by collagen. Dysplastic nevi were characterized by their increased intercellular distances and enlarged cell nuclei. Melanomas showed the presence of melanocytes and dendritic structures in all layers of the epidermis. Analysis of the metabolic state revealed that melanomas and dysplastic nevi were characterized by enhanced glycolysis. Optical coherence angiography shows that benign nevi had regular vascular networks and equal numbers of thin and thick vessels. Vascular networks of dysplastic nevi were characterized thin curved vessels. Thick irregular spiral vessels formed a dense microvascular network of melanomas.

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Elena V. Zagaynova, Vadim Elagin, Ekatarina Gubarkova, Oksana Garanina, Natalia Orlinskaya, Varvara V. Dudenkova, Irena Shlivko, Irina Klemenova, and Diana Davydova "Multiphoton imaging and OCT MA for diagnosis of human melanocytic lesions", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108820G (22 February 2019); https://doi.org/10.1117/12.2508480

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KEYWORDS

Melanoma

Fluorescence lifetime imaging

Optical coherence tomography

Angiography

Coherence (optics)

Luminescence

Multiphoton tomography

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