High spatial resolution and large field of view mobile phone-based microscopy with adaptive phone screen illumination (Conference Presentation)

Sara Kheireddine; Ayyappasamy Sudalaiyadum Perumal; Dan V. Nicolau; Sebastian Wachsmann-Hogiu

doi:10.1117/12.2510106

13 March 2019 High spatial resolution and large field of view mobile phone-based microscopy with adaptive phone screen illumination (Conference Presentation)

Sara Kheireddine, Ayyappasamy Sudalaiyadum Perumal, Dan V. Nicolau, Sebastian Wachsmann-Hogiu

Author Affiliations +

Proceedings Volume 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII; 108810M (2019) https://doi.org/10.1117/12.2510106
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Lens-based imaging approaches are faced with a trade-off between resolution and field-of-view (FOV). Generally, the greater the resolvable detail in a sample, the smaller the FOV we can observe. In our lab, we study the behaviour of microorganisms within confined spaces using microfluidic devices. In order to capture the full scope of their behaviour, we need to be able to discern individual microorganisms as well as observe the full microfluidic device area in real time. As such, visualizing such systems can be challenging, since we require an imaging system that can provide a resolution as high as 1 um, with a FOV large enough to fit our region of interest. To that end, we used the Nokia Lumia 1020 mobile phone, which has a 41.3 megapixel (MP) image sensor with a pixel size of 1.14 um, with an external lens attached to the camera for better focus, and we characterized the imaging system to have a spatial resolution of 1.2 um, with a FOV of 3.6 x 2.7 mm, and a working distance of 0.6 mm. Moreover, we used the screen of a Retina display Apple device as a versatile illumination source for this system. The screen is used to project various illumination patterns onto the specimen being imaged, each corresponding to a different illumination mode, with the Nokia phone capturing the resulting image. We tested our system by using it to image microorganisms such as Escherichia coli and Euglena gracilis within our microfluidic devices.

Conference Presentation

Citation Download Citation

Sara Kheireddine, Ayyappasamy Sudalaiyadum Perumal, Dan V. Nicolau, and Sebastian Wachsmann-Hogiu "High spatial resolution and large field of view mobile phone-based microscopy with adaptive phone screen illumination (Conference Presentation)", Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 108810M (13 March 2019); https://doi.org/10.1117/12.2510106

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Cell phones

Spatial resolution

Microscopy

Imaging systems

Microfluidics

Microorganisms

Image resolution

Show All Keywords

Keywords/Phrases

Search In:

Publication Years