MATLAB for laser speckle contrast analysis (LASCA): a practice-based approach

Eugene B. Postnikov; Maria O. Tsoy; Dmitry E. Postnov

doi:10.1117/12.2315189

26 April 2018 MATLAB for laser speckle contrast analysis (LASCA): a practice-based approach

Eugene B. Postnikov, Maria O. Tsoy, Dmitry E. Postnov

Proceedings Volume 10717, Saratov Fall Meeting 2017: Laser Physics and Photonics XVIII; and Computational Biophysics and Analysis of Biomedical Data IV; 1071728 (2018) https://doi.org/10.1117/12.2315189
Event: Saratov Fall Meeting 2017, 2017, Saratov, Russian Federation

Abstract

Laser Speckle Contrast Analysis (LASCA) is one of the most powerful modern methods for revealing blood dynamics. The experimental design and theory for this method are well established, and the computational recipie is often regarded to be trivial. However, the achieved performance and spatial resolution may considerable differ for different implementations. We comprise a minireview of known approaches to the spatial laser speckle contrast data processing and their realization in MATLAB code providing an explicit correspondence to the mathematical representation, a discussion of available implementations. We also present the algorithm based on the 2D Haar wavelet transform, also supplied with the program code. This new method provides an opportunity to introduce horizontal, vertical and diagonal speckle contrasts; it may be used for processing highly anisotropic images of vascular trees. We provide the comparative analysis of the accuracy of vascular pattern detection and the processing times with a special attention to details of the used MATLAB procedures.

Citation Download Citation

Eugene B. Postnikov, Maria O. Tsoy, and Dmitry E. Postnov "MATLAB for laser speckle contrast analysis (LASCA): a practice-based approach", Proc. SPIE 10717, Saratov Fall Meeting 2017: Laser Physics and Photonics XVIII; and Computational Biophysics and Analysis of Biomedical Data IV, 1071728 (26 April 2018); https://doi.org/10.1117/12.2315189

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