Raman imaging of biofilms using gold sputtered fiber optic probes

Christina Grace Charlet Christopher; Hariharan Manoharan; Aryasomayajula Subrahmanyam; V. V. Raghavendra Sai

doi:10.1117/12.2242973

9 December 2016 Raman imaging of biofilms using gold sputtered fiber optic probes

Christina Grace Charlet Christopher, Hariharan Manoharan, Aryasomayajula Subrahmanyam, V. V. Raghavendra Sai

Author Affiliations +

Proceedings Volume 10013, SPIE BioPhotonics Australasia; 100130D (2016) https://doi.org/10.1117/12.2242973
Event: SPIE BioPhotonics Australasia, 2016, Adelaide, Australia

Abstract

In this work we report characterization of bacterial biofilm using gold sputtered optical fiber probe as substrates for confocal Raman spectroscopy measurements. The chemical composition and the heterogeneity of biofilms in the extracellular polymeric substances (EPS) was evaluated. The spatial distribution of bacterial biofilm on the substrates during their growth phase was studied using Raman imaging. Further, the influence of substrate’s surface on bacterial adhesion was investigated by studying growth of biofilms on surfaces with hydrophilic and hydrophobic coatings. This study validates the use of gold sputtered optical fiber probes as SERS substrates in confocal microscopic configuration to identify and characterize clinically relevant biofilms.

Citation Download Citation

Christina Grace Charlet Christopher, Hariharan Manoharan, Aryasomayajula Subrahmanyam, and V. V. Raghavendra Sai "Raman imaging of biofilms using gold sputtered fiber optic probes ", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100130D (9 December 2016); https://doi.org/10.1117/12.2242973

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