Ultrabright plasmon-enhanced fluorescence for single-molecule biosensing

Peter Zijlstra

doi:10.1117/12.3003748

13 March 2024 Ultrabright plasmon-enhanced fluorescence for single-molecule biosensing

Peter Zijlstra

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550V (2024) https://doi.org/10.1117/12.3003748
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

I will describe the use of plasmon-enhanced fluorescence to boost single-molecule fluorescence signals by more than two orders of magnitude. I will discuss two applications of this approach that directly exploit these ultrabright signals. Firstly, they enable highly sensitive single-molecule DNA diagnostics directly in serum on an affordable and miniaturized microscopy platform. By using a reversible sandwich assay I will describe a continuous monitoring biosensor that is suitable for point-of-care diagnostics. Secondly, the ultrabright signals pave the way to real-time probing of single-molecule dynamics on so-far inaccessible microsecond timescales. I will show recent results where we study the dynamics of monovalent and multivalent DNA constructs in real time with microsecond integration times.

Conference Presentation

Citation Download Citation

Peter Zijlstra "Ultrabright plasmon-enhanced fluorescence for single-molecule biosensing", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550V (13 March 2024); https://doi.org/10.1117/12.3003748

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