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20 May 2022 Smart scanning fluorescent microscope for cell sheet imaging
Faris Abouakil, Huicheng Meng, Marie-Anne Burcklen, Hervé Rigneault, Loic LeGoff, Frédéric Galland
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Proceedings Volume PC12136, Unconventional Optical Imaging III; PC1213603 (2022) https://doi.org/10.1117/12.2621590
Event: SPIE Photonics Europe, 2022, Strasbourg, France
Abstract
Confocal fluorescence microscopy is a privileged tool for life imaging, but can generate phototoxicity due to the prolonged sample illumination. When cells are organized along sheets lying on 2D surfaces curved in a 3D volume (e.g. epithelial cells), we propose a new approach allowing to automatically estimate the surface on which these cells are distributed from a small number of acquisitions (typically 0.1% of the voxels). This allows to concentrate thereafter the illumination around the surface of interest and thus to scan only a small portion (typically between 1% and 5%) of the volume containing the sample.
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Faris Abouakil, Huicheng Meng, Marie-Anne Burcklen, Hervé Rigneault, Loic LeGoff, and Frédéric Galland "Smart scanning fluorescent microscope for cell sheet imaging", Proc. SPIE PC12136, Unconventional Optical Imaging III, PC1213603 (20 May 2022); https://doi.org/10.1117/12.2621590
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KEYWORDS
Microscopes
3D acquisition
Confocal microscopy
Luminescence
Microscopy
Natural surfaces
Laser induced fluorescence
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