From fluctuations to function: FFS to explore bio-molecular organisation

Falk Schneider; Pablo F. Cespedes; Erdinc Sezgin; Marco Fritzsche; Scott E. Fraser

doi:10.1117/12.2608907

2 March 2022 From fluctuations to function: FFS to explore bio-molecular organisation

Falk Schneider, Pablo F. Cespedes, Erdinc Sezgin, Marco Fritzsche, Scott E. Fraser

Proceedings Volume PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV; PC1196706 (2022) https://doi.org/10.1117/12.2608907
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Fluorescence fluctuation spectroscopy (FFS) offers a window to observe and investigate dynamics of bio-molecular organisation. Furthermore, FFS acquisitions uniquely allow obtaining insights into other properties of the sample such as concentration and brightness (counts per molecule) of the fluorescently labelled species simultaneously. Here, we exploit the large number of measurements and statistics obtained from scanning fluorescence fluctuation spectroscopy (sFFS) acquisitions to elucidate bio-molecular organisation. We demonstrate the versatility and sensitivity of the approach using computer simulations, synthetic biological membranes and living cells.

Conference Presentation

Citation Download Citation

Falk Schneider, Pablo F. Cespedes, Erdinc Sezgin, Marco Fritzsche, and Scott E. Fraser "From fluctuations to function: FFS to explore bio-molecular organisation", Proc. SPIE PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV, PC1196706 (2 March 2022); https://doi.org/10.1117/12.2608907

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