Antibody-target binding in living tumors using macroscopy fluorescence lifetime imaging

Margarida Barroso; Alena Rudkouskaya; Jason Smith; John Williams; Xavier Intes

doi:10.1117/12.2609024

7 March 2022 Antibody-target binding in living tumors using macroscopy fluorescence lifetime imaging

Margarida Barroso, Alena Rudkouskaya, Jason Smith, John Williams, Xavier Intes

Author Affiliations +

Proceedings Volume PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII; PC119650C (2022) https://doi.org/10.1117/12.2609024
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

There is a continuing need to develop preclinical molecular imaging modalities to guide the development and optimization of targeted therapies in oncology. We have established Macroscopic Fluorescence Lifetime Imaging (MFLI) associated with Forster Resonance Energy Transfer (FRET) to report quantitatively on antibody-target engagement in live intact animals at the organ level. Here, we use FLI FRET imaging to quantify the binding of near infrared labeled antibody drugs to breast cancer cells or tumor xenografts. In combination with antibody engineering, this approach provides a robust analytical tool to optimize the binding and uptake of antibody-target binding during tumor progression and treatment.

Conference Presentation

Citation Download Citation

Margarida Barroso, Alena Rudkouskaya, Jason Smith, John Williams, and Xavier Intes "Antibody-target binding in living tumors using macroscopy fluorescence lifetime imaging", Proc. SPIE PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII, PC119650C (7 March 2022); https://doi.org/10.1117/12.2609024

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE