Multiphoton microscopy of A. phagocytophilum infection

Alex J. Walsh; Vidhya Shree Ravi; Adela Oliva Chavez

doi:10.1117/12.3003301

13 March 2024 Multiphoton microscopy of A. phagocytophilum infection

Alex J. Walsh, Vidhya Shree Ravi, Adela Oliva Chavez

Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470M (2024) https://doi.org/10.1117/12.3003301
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Anaplasma phagocytophilum is the intracellular bacteria that causes Human Granulocytic Anaplasmosis (HGA). While the skin is known as the entry site for the bacteria, HGA is characterized by systemic infection of A. phagocytophilum, but it is unknown how a localized infection of A. phagocytophilum leads to HGA. Here, we use a multimodal microscopy approach to image collagen and immune cell alterations due to A. phagocytophilum infection. Second-harmonic generation imaging revealed collagen fiber density decreased at the tick bite of infected skin. Additionally, significant differences in autofluorescence lifetime were observed due to A. phagocytophilum infection of human myeloid HL-60 cells.

Conference Presentation

Citation Download Citation

Alex J. Walsh, Vidhya Shree Ravi, and Adela Oliva Chavez "Multiphoton microscopy of A. phagocytophilum infection", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470M (13 March 2024); https://doi.org/10.1117/12.3003301

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available