Comparing angular scattering-based organelle size estimates to tomography-based size estimates in single cells (Conference Presentation)

Kaitlin J. Dunn; George Funkenbusch; Andrew J. Berger

doi:10.1117/12.2648475

15 March 2023 Comparing angular scattering-based organelle size estimates to tomography-based size estimates in single cells (Conference Presentation)

Kaitlin J. Dunn, George Funkenbusch, Andrew J. Berger

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Proceedings Volume PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; PC123850G (2023) https://doi.org/10.1117/12.2648475
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Angular light scattering provides a promising method for sizing organelle distributions in single cells, but most methods of validating size estimates are ill-suited for single cells. We demonstrate tomography-based size estimates as a simultaneous, independent, non-destructive comparison. Optical Diffraction Tomography (ODT) builds a 3D refractive index map which is segmented to provide an estimate of organelle sizes. The normal incidence complex field measurement obtained as part of ODT is Fourier transformed to compute the angular scattering. Comparing both approaches will provide important insight into the capabilities of using angular light scattering to estimate organelle size distributions in single cells.

Conference Presentation

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Kaitlin J. Dunn, George Funkenbusch, and Andrew J. Berger "Comparing angular scattering-based organelle size estimates to tomography-based size estimates in single cells (Conference Presentation)", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC123850G (15 March 2023); https://doi.org/10.1117/12.2648475

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KEYWORDS

Mie scattering

Scattering

Light scattering

3D metrology

Refractive index

Optical tomography

Scatter measurement

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