Collinear ultrasonic and multiphoton focusing for advanced functional imaging and stimulation (Conference Presentation)

Justin Little; Behnam Tayebi; Yi Yuan; Michal Balberg; Shy Shoham

doi:10.1117/12.2650811

15 March 2023 Collinear ultrasonic and multiphoton focusing for advanced functional imaging and stimulation (Conference Presentation)

Justin Little, Behnam Tayebi, Yi Yuan, Michal Balberg, Shy Shoham

Author Affiliations +

Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC123840R (2023) https://doi.org/10.1117/12.2650811
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Ultrasonic methods hold immense promise for functional measurements and interventions during in vivo studies due to the beneficial scattering properties of acoustic waves. Here we present the design and application of a system that integrates into standard multiphoton microscopes, which co-linearly combines two-photon fluorescence imaging and precise acoustic sonication. By applying this system across a range of samples, we observed a novel gigantic Acousto-Optic (AO) modulation of two-photon excited fluorescence, and also study the mechanisms of functional ultrasound neuromodulation, an emerging non-invasive neurostimulation technique. This work demonstrates simple photonic/acoustic methods to study novel phenomenon in the awake mouse.

Conference Presentation

Citation Download Citation

Justin Little, Behnam Tayebi, Yi Yuan, Michal Balberg, and Shy Shoham "Collinear ultrasonic and multiphoton focusing for advanced functional imaging and stimulation (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840R (15 March 2023); https://doi.org/10.1117/12.2650811

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