Biophysical measurements of subcellular dynamics

Nada N. Boustany

doi:10.1117/12.2615348

3 March 2022 Biophysical measurements of subcellular dynamics

Nada N. Boustany

Proceedings Volume PC11974, Biomedical Applications of Light Scattering XII; PC1197409 (2022) https://doi.org/10.1117/12.2615348
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Dynamic changes in cellular morphology and subcellular structure are the result of underlying biochemical activity and molecular signaling pathways controlling cellular function. I will present two cellular imaging methods that directly encode and quantify the morphological and physical properties of cells. First, I present optical scatter imaging as a means of tracking sub-wavelength changes in organelle morphology in situ. We have conceived, developed and used this label-free technique to quantify mitochondrial fission in response intracellular calcium overload and apoptosis. Then, I present the prospects of utilizing a fluorescent molecular tension probe to map intracellular forces. We are currently utilizing this technique to study dendrtitic branching in neuronal cells. Our ultimate goal is to combine these microscopic technologies to enable the discovery of novel physical markers that will enhance the quantitative analysis and diagnosis of cellular function.

Conference Presentation

Citation Download Citation

Nada N. Boustany "Biophysical measurements of subcellular dynamics", Proc. SPIE PC11974, Biomedical Applications of Light Scattering XII, PC1197409 (3 March 2022); https://doi.org/10.1117/12.2615348

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