High speed autofluorescence imaging of the effects of short pulses of infrared light

Alex J. Walsh; Jocelyn Martinez; Anna Theodossiou

doi:10.1117/12.2613561

2 March 2022 High speed autofluorescence imaging of the effects of short pulses of infrared light

Alex J. Walsh, Jocelyn Martinez, Anna Theodossiou

Proceedings Volume PC11971, High-Speed Biomedical Imaging and Spectroscopy VII; PC119710B (2022) https://doi.org/10.1117/12.2613561
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Imaging the spatial and temporal effects of millisecond duration pulses of infrared light on neurons requires image frame rates approaching 1000+ Hz to capture neural activity. Autofluorescence imaging of the metabolic coenzymes reduced nicotinamide adenine dinucleotide and flavin adenine dinucleotide provides information about cellular metabolism and can be a surrogate measurement of neural activity. Here, we are combining fast fluorescence microscopy techniques with modeling and machine learning to image autofluorescence dynamics in cells following exposure to infrared light.

Conference Presentation

Citation Download Citation

Alex J. Walsh, Jocelyn Martinez, and Anna Theodossiou "High speed autofluorescence imaging of the effects of short pulses of infrared light", Proc. SPIE PC11971, High-Speed Biomedical Imaging and Spectroscopy VII, PC119710B (2 March 2022); https://doi.org/10.1117/12.2613561

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