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2 March 2022 Scattering correcting wavefront shaping for three-photon microscopy
Bernhard Rauer, Laurent Bourdieu, Hilton Barbosa de Aguiar, Sylvain Gigan
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Proceedings Volume PC11969, Adaptive Optics and Wavefront Control for Biological Systems VIII; PC1196902 (2022) https://doi.org/10.1117/12.2609561
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
The main obstacle for optical imaging deep inside biological tissues is light scattering. Recently, three-photon (3p) microscopy extended the accessible depth ranges of fluorescence imaging due to its enhanced nonlinearity. On a different front, advances in optical wavefront shaping showed that scattering can be compensated for, even in regimes where light entirely lost its directionality. Combining these two approaches, we demonstrate focusing and imaging behind scattering layers, enabled through wavefront shaping guided by 3p fluorescence in situations where no ballistic light reaches the sample. We analyse different sample geometries and compare these results to the case of 2p excitation.
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Bernhard Rauer, Laurent Bourdieu, Hilton Barbosa de Aguiar, and Sylvain Gigan "Scattering correcting wavefront shaping for three-photon microscopy", Proc. SPIE PC11969, Adaptive Optics and Wavefront Control for Biological Systems VIII, PC1196902 (2 March 2022); https://doi.org/10.1117/12.2609561
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KEYWORDS
Light scattering
Microscopy
Scattering
Wavefronts
Multiphoton microscopy
Tissue optics
Luminescence
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