Fast light sculpting techniques for online optical interrogation of biological functions

Nicolas C. Pégard

doi:10.1117/12.2616203

7 March 2022 Fast light sculpting techniques for online optical interrogation of biological functions

Nicolas C. Pégard

Proceedings Volume PC11944, Multiscale Imaging and Spectroscopy III; PC1194409 (2022) https://doi.org/10.1117/12.2616203
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Sculpting light in 3D at high speeds is critical to track and manipulate biological events at cellular scales in real-time. Yet, even the current state of the art, Computer-Generated Holography (CGH), operates with slow algorithms and does not provide enough degrees of control to focus light precisely through deep biological tissue. We address these two challenges with new hardware and algorithms. First, we introduce DeepCGH, a deep learning model that synthesizes 3D holograms in milliseconds, and we demonstrate experimental benefits in multiphoton microscopy applications. We then present new optical instruments that modulate light both in space and time to render 4D light fields with much greater fidelity than coherent CGH techniques.

Conference Presentation

Citation Download Citation

Nicolas C. Pégard "Fast light sculpting techniques for online optical interrogation of biological functions", Proc. SPIE PC11944, Multiscale Imaging and Spectroscopy III, PC1194409 (7 March 2022); https://doi.org/10.1117/12.2616203

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