In vivo two-photon imaging measuring the blood-brain barrier permeability during early postnatal brain development in rodent

Lingyan Shi; Adrián Rodríguez-Contreras

doi:10.1117/12.2219571

14 March 2016 In vivo two-photon imaging measuring the blood-brain barrier permeability during early postnatal brain development in rodent

Lingyan Shi, Adrián Rodríguez-Contreras

Author Affiliations +

Proceedings Volume 9712, Multiphoton Microscopy in the Biomedical Sciences XVI; 97121Z (2016) https://doi.org/10.1117/12.2219571
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The blood–brain barrier (BBB) is a unique structure between the cerebral blood circulation and the delicate neural environment that is important in regulating the movement of molecules and ions involved in brain development and function. However, little is known about the physiological permeability of molecules and ions across the BBB during brain development. In this study we applied an innovative approach to examine the development of BBB properties quantitatively. Two-photon microscopy was employed to measure BBB permeability in real time in vivo. Vascular growth and specific interactions between astrocyte end feet and microvessels were studied by using a combination of IB4 histochemistry, immunohistochemistry, confocal microscopy and 3D analysis.

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Lingyan Shi and Adrián Rodríguez-Contreras "In vivo two-photon imaging measuring the blood-brain barrier permeability during early postnatal brain development in rodent", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97121Z (14 March 2016); https://doi.org/10.1117/12.2219571

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