Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy

A. Jouonang; F. Przybilla; J. Godet; K. K. Sharma; T. Restlé; H. de Rocquigny; J.-L. Darlix; C. Kenfack; P. Didier; Y. Mély

doi:10.1117/12.2003478

22 February 2013 Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy

A. Jouonang, F. Przybilla, J. Godet, K. K. Sharma, T. Restlé, H. de Rocquigny, J.-L. Darlix, C. Kenfack, P. Didier, Y. Mély

Author Affiliations +

Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI; 859009 (2013) https://doi.org/10.1117/12.2003478
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

During reverse transcription, the HIV-1 RNA is converted by the reverse transcriptase (RT) into proviral DNA. RT is assisted by the HIV-1 nucleocapsid (NCp7) protein that notably increases the ability of RT to synthesize DNA through pause sites. Using single molecule FRET, we monitored the NCp7 effect on the binding of RT to nucleic acid sequences corresponding to two different pause sites. NCp7 was found to modify the distribution of RT orientations on the oligonucleotides and decrease the residence time of RT on one of the pause sites. These results give direct insight into the NCp7 molecular mechanism in reverse transcription.

Citation Download Citation

A. Jouonang, F. Przybilla, J. Godet, K. K. Sharma, T. Restlé, H. de Rocquigny, J.-L. Darlix, C. Kenfack, P. Didier, and Y. Mély "Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy", Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 859009 (22 February 2013); https://doi.org/10.1117/12.2003478

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available