Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization

Hod Dana; Anat Marom; Nimrod Kruger; Aviv Ellman; Shy Shoham

doi:10.1117/12.908844

9 February 2012 Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization

Hod Dana, Anat Marom, Nimrod Kruger, Aviv Ellman, Shy Shoham

Author Affiliations +

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 822603 (2012) https://doi.org/10.1117/12.908844
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The development of rapid volumetric imaging systems for functional multiphoton microscopy is essential for dynamical imaging of large-scale neuronal network activity. Here, we introduce a line-illuminating temporal-focusing microscope capable of rapid three-dimensional imaging at 10-20 volumes/sec, and study the system's characteristics both theoretically and experimentally. We demonstrate that our system is capable of functional volumetric calcium imaging of distributed neuronal activity patterns, and introduce a computational strategy for activity reconstruction in strongly scattering media.

Citation Download Citation

Hod Dana, Anat Marom, Nimrod Kruger, Aviv Ellman, and Shy Shoham "Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822603 (9 February 2012); https://doi.org/10.1117/12.908844

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