Quantum dot-fluorescent protein pair as ratiometric pH sensor

Allison M. Dennis; Gang Bao

doi:10.1117/12.842640

17 February 2010 Quantum dot-fluorescent protein pair as ratiometric pH sensor

Allison M. Dennis, Gang Bao

Proceedings Volume 7575, Colloidal Quantum Dots for Biomedical Applications V; 75750C (2010) https://doi.org/10.1117/12.842640
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Fluorescence resonance energy transfer (FRET) between a quantum dot (QD) and the pH-sensitive fluorescent protein mOrange has been used to develop a fluorescent pH-indicator that is bright and photostable enough for applications in fluorescence imaging, including the tracking of molecules through endocytic pathways. As the molar extinction coefficient of mOrange increases with pH, the ratio of the mOrange emission to the QD emission (F_A/F_D) increases sharply, producing greater than 10-fold increases in the F_A/F_D ratio between pH 4.5 and 7.5. This probe has been thoroughly characterized and it intracellular imaging potential explored.

Citation Download Citation

Allison M. Dennis and Gang Bao "Quantum dot-fluorescent protein pair as ratiometric pH sensor", Proc. SPIE 7575, Colloidal Quantum Dots for Biomedical Applications V, 75750C (17 February 2010); https://doi.org/10.1117/12.842640

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