Turn-key calibration of counter-propagating multiple beam 3D trapping system

Jeppe Seidelin Dam; Ivan R. Perch-Nielsen; Darwin Palima; Jesper Glückstad

doi:10.1117/12.763096

5 March 2008 Turn-key calibration of counter-propagating multiple beam 3D trapping system

Jeppe Seidelin Dam, Ivan R. Perch-Nielsen, Darwin Palima, Jesper Glückstad

Proceedings Volume 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI; 68590Z (2008) https://doi.org/10.1117/12.763096
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Optical trapping by use of multiple counter-propagating beam traps has not been widely implemented outside optical engineering laboratories. One, if not the primary, reason for this is the relatively complex calibration procedures involved in connection with this optical geometry. In this talk, we present automated solutions to all the calibration issues, which in effect results in a turn-key counter-propagating multi-beam 3D trapping system. These results allow a wider audience to utilize counter-propagating beam trapping systems. The calibrated system can be used to independently manipulate a plurality of cells real-time in a large 3D working area. Optionally, the system can be extended to allow for use of various spectroscopic methods concurrently with optical manipulation/trapping.

Citation Download Citation

Jeppe Seidelin Dam, Ivan R. Perch-Nielsen, Darwin Palima, and Jesper Glückstad "Turn-key calibration of counter-propagating multiple beam 3D trapping system", Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 68590Z (5 March 2008); https://doi.org/10.1117/12.763096

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KEYWORDS

Mirrors

Calibration

Reticles

Cameras

Spatial light modulators

Optical tweezers

Image analysis

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