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The Aminolevulinic Acid (ALA) - Protoporphyrin IX (PpIX) system is unique in the world of photosensitizers in that
the prodrug ALA is enzymatically transformed via the tissue of interest into fluorescently detectable levels of PpIX.
This system can be used to monitor cellular metabolism of tumor tissue for applications such as therapy monitoring.
Detecting PpIX fluorescence noninvasively has proven difficult due to the high levels of PpIX produced in the skin
compared to other tissue both with and without ALA administration. In the current study, methods to decrease skin
PpIX autofluorescence and skin PpIX fluorescence following ALA administration have been examined. Use of a
purified diet is found to decrease both skin PpIX autofluorescence and skin PpIX fluorescence following ALA
administration, while addition of a broad spectrum antibiotic to the water shows little effect. Following ALA
administration, improved brain tumor detection is seen when skin PpIX fluorescence is photobleached via blue light
prior to transmission spectroscopic measurements of tumor bearing and control animals. Both of these methods to
decrease skin PpIX autofluorescence and skin PpIX fluorescence following ALA administration are shown to have a
large effect on the ability to detect tumor tissue PpIX fluorescence noninvasively in vivo.
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Summer L. Gibbs, Julia A. O'Hara, P. Jack Hoopes, Brian W. Pogue, "Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence," Proc. SPIE 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI, 64270D (27 February 2007); https://doi.org/10.1117/12.700456