Analysis of the effect on tissue autofluorescence detection from off-focus of linear-array pinholes in LP-laser scanning confocal fluorescence microscopical imaging spectrometer

Xing Su; Weijian Tian; Chunmin Zhang

doi:10.1117/12.667176

2 February 2006 Analysis of the effect on tissue autofluorescence detection from off-focus of linear-array pinholes in LP-laser scanning confocal fluorescence microscopical imaging spectrometer

Xing Su, Weijian Tian, Chunmin Zhang

Author Affiliations +

Proceedings Volume 6026, ICO20: Biomedical Optics; 60260S (2006) https://doi.org/10.1117/12.667176
Event: ICO20:Optical Devices and Instruments, 2005, Changchun, China

Abstract

The research of antofluorescence of the live tissue starts to be used in the disease diagnoses and prevention in recent years. The laser scanning confocal microscope has always played a great role in the autofluorescence research in biomedical field. As the technology of imaging spectrometer got successfully used in the remote sensing fields, microscopical imaging spectrometer for biomedical analyses emerged after the integration of imaging spectrometer and laser scanning confocal microscope. It can get the autofluorescence image and the spectral information of the sample simultaneously, this will supply more information to the diagnoses for difficult diseases. In this paper the principle of a Linear-array Pinholes Laser Scanning Confocal Microscopical Imaging Spectrometer (LP-LSCMIS) developed by the authors is proposed. It can be widely used in the autofluorescence detection of the live tissue in the body, such as the gastric, kidney, etc. The effect of off-focus of linear-array pinholes on microscopical imaging spectrometer subsystem in this device is mainly analyzed. The spatially resolution of fluorescence imaging and the modulation depth of autofluorescence spectrum in the live tissue is affected by the off-focus according to our research. We analyse the modulation depth of the interferogram thinking of the factor of off-focus.

Citation Download Citation

Xing Su, Weijian Tian, and Chunmin Zhang "Analysis of the effect on tissue autofluorescence detection from off-focus of linear-array pinholes in LP-laser scanning confocal fluorescence microscopical imaging spectrometer", Proc. SPIE 6026, ICO20: Biomedical Optics, 60260S (2 February 2006); https://doi.org/10.1117/12.667176

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