Imaging brain morphology with ultrahigh-resolution optical coherence tomography

Kostadinka K. Bizheva; Angelika Unterhuber; Boris Hermann; Boris Povazay; Harald Sattmann; Michael Mei; Ronald Holzwarth; Matthias Preusser; Herbert Reitsamer; Michael Seefeldt; Ralf Menzel; Herbert Budka; Adolf Friedrich Fercher; Wolfgang Drexler

doi:10.1117/12.501164

2 October 2003 Imaging brain morphology with ultrahigh-resolution optical coherence tomography

Kostadinka K. Bizheva, Angelika Unterhuber, Boris Hermann, Boris Povazay, Harald Sattmann, Michael Mei, Ronald Holzwarth, Matthias Preusser, Herbert Reitsamer, Michael Seefeldt, Ralf Menzel, Herbert Budka, Adolf Friedrich Fercher, Wolfgang Drexler

Author Affiliations +

Proceedings Volume 5140, Optical Coherence Tomography and Coherence Techniques; (2003) https://doi.org/10.1117/12.501164
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

The morphology of healthy and pathological human brain tissue, as well as the brain structural organization of various animal models has been imaged in-vitro using ultrahigh resolution optical coherence tomography (UHR OCT). Micrometer-scale OCT resolution (< 2 μm axial resolution) was achieved at different central wavelengths by interfacing three state-of-the-art broad bandwidth light sources (Ti:Al₂O₃, λc = 790 nm, Δλ = 260 nm and P_out = 50 mW; PCF based laser, λc = 1150 nm, Δλ = 350 nm and P_out = 2 W; Fiber laser based light source, λc = 1350 nm, Δλ = 470 nm and P_out = 4 mW) to a modular free-space OCT system, utilizing a dynamic focusing and designed for optimal performance in the appropriate wavelength regions. Images acquired from a fixed honeybee brain demonstrated the ability of UHR OCT to image the globular structure of the brain, some fine morphological details such as the nerve fiber bundles connecting the medulla (visual center) to the honeybee eyes, and the interfaces between different tissue layers in the medulla. Tomograms of various human neuropathologies demonstrated the feasibility of UHR OCT to visualize morphological details such as small (~20 μm) calcifications typical for fibrous meningioma, and enlarged nuclei of cancer cells (~10-15 μm) characteristic for many other neuropathologies. In addition UHR OCT was used to image cellular morphology in living ganglion cells.

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Kostadinka K. Bizheva, Angelika Unterhuber, Boris Hermann, Boris Povazay, Harald Sattmann, Michael Mei, Ronald Holzwarth, Matthias Preusser, Herbert Reitsamer, Michael Seefeldt, Ralf Menzel, Herbert Budka, Adolf Friedrich Fercher, and Wolfgang Drexler "Imaging brain morphology with ultrahigh-resolution optical coherence tomography", Proc. SPIE 5140, Optical Coherence Tomography and Coherence Techniques, (2 October 2003); https://doi.org/10.1117/12.501164

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