Practical guide for constructing a pulse compressor used in multiphoton microscopy

James D. Lechleiter; Da-Ting Lin; Ilse Sienaert

doi:10.1117/12.424543

24 April 2001 Practical guide for constructing a pulse compressor used in multiphoton microscopy

James D. Lechleiter, Da-Ting Lin, Ilse Sienaert

Proceedings Volume 4262, Multiphoton Microscopy in the Biomedical Sciences; (2001) https://doi.org/10.1117/12.424543
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Multi-photon imaging has generated intense interest from investigators using fluorescent imaging assays in live cells. It can be argued that this technique is rapidly overtaking confocal microscopy as the method of choice for three- dimensional fluorescent imaging of in vivo preparations. Because of the cost involved in purchasing a commercial multi- photon imaging microscope, many investigators have elected to build their own system by adapting in-house confocal laser scanning microscopes. One of the components used for this adaptation involves the construction of an external pulse compressor. Pulse compressors are used to add negative dispersion to pulsed radiation, which undergoes group velocity dispersion when traversing optical elements. In this chapter, we describe a practical guide to building an external pulse compressor.

Citation Download Citation

James D. Lechleiter, Da-Ting Lin, and Ilse Sienaert "Practical guide for constructing a pulse compressor used in multiphoton microscopy", Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); https://doi.org/10.1117/12.424543

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