Spectral measurements of dc potentials in perfused rat liver by the use of Merocyanine-540

Christine Mahlke; Markus Boehnert; Robert Rauh M.D.; Manfred D. Kessler

doi:10.1117/12.431558

4 May 2001 Spectral measurements of dc potentials in perfused rat liver by the use of Merocyanine-540

Christine Mahlke, Markus Boehnert, Robert Rauh M.D., Manfred D. Kessler

Proceedings Volume 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II; (2001) https://doi.org/10.1117/12.431558
Event: Saratov Fall Meeting 2000, 2001, Saratov, Russian Federation

Abstract

In organs we can find signal changes between vascular and parenchymal cells. We started to combine spectral measurements by the use of merocyanine in isolated perfused rat liver to analyze the alterations of dc-potentials. There have been first experiments with Merocyanine (M-540) in the seventies with stained axons and hearts to measure optical action potentials (Morad, Salama, et al, 1978). It is reported that the increase of fluorescence response is connected to a depolarization and its decrease to a repolarization. Tissue imaging after staining with this dye should be ideal for a long term interpretation of dc-potential alterations as an analysis of the electrical coupling in different cell types under various experimental conditions like anoxia or temperature changes.

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Christine Mahlke, Markus Boehnert, Robert Rauh M.D., and Manfred D. Kessler "Spectral measurements of dc potentials in perfused rat liver by the use of Merocyanine-540", Proc. SPIE 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II, (4 May 2001); https://doi.org/10.1117/12.431558

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KEYWORDS

Liver

Luminescence

Tissues

Action potentials

Heart

Tissue optics

Axons

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