Measurement of in-vivo hemoglobin concentration using diffuse reflectance

Ashley D. Hunter; John A. Crowe; John G. Walker

doi:10.1117/12.334355

30 December 1998 Measurement of in-vivo hemoglobin concentration using diffuse reflectance

Ashley D. Hunter, John A. Crowe, John G. Walker

Proceedings Volume 3566, Photon Propagation in Tissues IV; (1998) https://doi.org/10.1117/12.334355
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

A means of determining the concentration of hemoglobin in- vivo using diffuse reflectance is proposed which could be applied to the quantification of laser Doppler flowmetry data. Monte Carlo simulations were used to generate intensity vs. source-detector separation plots for optical fibers of 200 micrometers diameter, with separations up to 6 mm on the skin surface. The plots were then fitted to the equation; I(x) equals a₀.exp(-a₁x + a₂x²) and a polynomial function derived for a₁ that is dependent upon the reduced scattering, microsecond(s) ', and absorption, (mu) a, coefficients. Experimentally two wavelengths with a known difference in the absorption coefficient of hemoglobin, but sharing the same microsecond(s) ', are used to solve simultaneously the derived function for (mu) a, and give the hemoglobin concentration.

Citation Download Citation

Ashley D. Hunter, John A. Crowe, and John G. Walker "Measurement of in-vivo hemoglobin concentration using diffuse reflectance", Proc. SPIE 3566, Photon Propagation in Tissues IV, (30 December 1998); https://doi.org/10.1117/12.334355

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