Photodynamic treatment with zinc poly-substituted phthalocyanines causes the release of tissue factor in an endothelial cell line

Janet E. Cruse-Sawyer; B. Dixon; John Griffiths; Stanley B. Brown

doi:10.1117/12.260757

4 December 1996 Photodynamic treatment with zinc poly-substituted phthalocyanines causes the release of tissue factor in an endothelial cell line

Janet E. Cruse-Sawyer, B. Dixon, John Griffiths, Stanley B. Brown

Author Affiliations +

Proceedings Volume 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II; (1996) https://doi.org/10.1117/12.260757
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Tissue factor (TF) is not expressed by endothelial cells unless they have ben perturbed. Identifying the extrinsic pathway of coagulation as the process involved in clot formation demonstrated that PDT induces release of TF by endothelial cells. EAhy 926 cells were incubated with 10 (mu) g ml^-1 of photosensitizer for 24 h prior to illumination at 40 J cm^-2. A clotting assay was then performed. PDT caused a significant reduction in clotting time. This was repeated using factor VII- or X-deficient serum. In factor VII-deficient serum there was no difference between treated and untreated cells. There was no clot formation with any cells in the presence of factor x- deficient serum. The data indicate that PDT-induced procoagulation activity is via the extrinsic pathway of coagulation and results from direct initiation of endothelial cells to release tissue factor.

Citation Download Citation

Janet E. Cruse-Sawyer, B. Dixon, John Griffiths, and Stanley B. Brown "Photodynamic treatment with zinc poly-substituted phthalocyanines causes the release of tissue factor in an endothelial cell line", Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); https://doi.org/10.1117/12.260757

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