Uptake, subcellular localization, and phototoxicity of photosensitizing porphyrins

Herbert Schneckenburger; Reinhard Sailer; Michael H. Gschwend; Karin Kunzi-Rapp; Nermin Akguen; Inna Tregub; Wolfgang S. L. Strauss

doi:10.1117/12.230975

31 January 1996 Uptake, subcellular localization, and phototoxicity of photosensitizing porphyrins

Herbert Schneckenburger, Reinhard Sailer, Michael H. Gschwend, Karin Kunzi-Rapp, Nermin Akguen, Inna Tregub, Wolfgang S. L. Strauss

Author Affiliations +

Proceedings Volume 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities; (1996) https://doi.org/10.1117/12.230975
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

Cytotoxicity, uptake, intracellular location and phototoxicity were compared for the naturally occurring porphyrins uroporphyrin III (UP), coproporphyrin III (CP) and protoporphyrin IX (PP), as well as for tetra-, tri-, di- and monosulphonated meso-tetraphenyl porphyrins (TPPS_n with n equals 4, 3, 2a, 1) in cultivated endothelial cells from calf aorta (BKEz-7). In addition, the uptake of CP and PP in the chick chorioallantoic membrane (CAM) was studied after intravenous application. Hydrophilic porphyrins (UP, CP, TPPS₄ and TPPS₃) exhibit low cellular uptake as well as low cyto- and phototoxicity as compared with the more hydrophobic compounds. UP, CP, TPPS₄ and TPPS₃ were mainly located in lysosomes, whereas PP, TPPS_2a and TPPS₁ were preferentially observed in the cytoplasm -- supposed to be located in cellular membranes of organelles -- and exhibited pronounced light-induced reactions. A rapid uptake by the CAM vasculature (5 min. - 30 min. after application) and some delayed uptake by the CAM matrix (30 min. - 6 h with a maximum at 60 min.) were measured for CP and PP.

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Herbert Schneckenburger, Reinhard Sailer, Michael H. Gschwend, Karin Kunzi-Rapp, Nermin Akguen, Inna Tregub, and Wolfgang S. L. Strauss "Uptake, subcellular localization, and phototoxicity of photosensitizing porphyrins", Proc. SPIE 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities, (31 January 1996); https://doi.org/10.1117/12.230975

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