Determination of absolute concentration of oxy- and deoxyhemoglobin in rat head by time-resolved Beer-Lambert law

Motoki Oda; Yutaka Yamashita; Goro Nishimura; Mamoru Tamura

doi:10.1117/12.209948

30 May 1995 Determination of absolute concentration of oxy- and deoxyhemoglobin in rat head by time-resolved Beer-Lambert law

Motoki Oda, Yutaka Yamashita, Goro Nishimura, Mamoru Tamura

Author Affiliations +

Proceedings Volume 2389, Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation; (1995) https://doi.org/10.1117/12.209948
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Time-resolved absorption spectra were measured in the near-IR wavelength range from 700 to 900 nm with rat heads in vivo under various respiratory conditions, based on our time- resolved microscopic Beer-Lambert law. The spectra were expressed in absolute absorption per unit optical path (cm^-1). Applying a regression analysis to the spectra, we obtained absolute concentrations of HbO₂ and Hb in the rat head. These values agreed with those measured in direct assay analysis, and the feasibility of our time-resolved photometry for quantitation of chromophores in living tissue was demonstrated. This fact supports our assumption that light absorption in a scattering media such as living tissues in independent of the scattering. Based on this study, we constructed a portable system for clinical applications. The system employs three diode lasers of different wavelengths for generating picosecond light pulses and high speed PMT for a time-correlated single photon counting method. The system performance shows its applicability to quantitative monitoring of oxygen metabolism in living tissues.

Citation Download Citation

Motoki Oda, Yutaka Yamashita, Goro Nishimura, and Mamoru Tamura "Determination of absolute concentration of oxy- and deoxyhemoglobin in rat head by time-resolved Beer-Lambert law", Proc. SPIE 2389, Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation, (30 May 1995); https://doi.org/10.1117/12.209948

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