Optical detection of parasitic protozoa in sol-gel matrices

Jacques Livage; J. Y. Barreau; J. M. Da Costa; I. Desportes

doi:10.1117/12.188984

13 October 1994 Optical detection of parasitic protozoa in sol-gel matrices

Jacques Livage, J. Y. Barreau, J. M. Da Costa, I. Desportes

Proceedings Volume 2288, Sol-Gel Optics III; (1994) https://doi.org/10.1117/12.188984
Event: SPIE's 1994 International Symposium on Optics, Imaging, and Instrumentation, 1994, San Diego, CA, United States

Abstract

Whole cell parasitic protozoa have been entrapped within sol-gel porous silica matrices. Stationary phase promastigote cells of Leishmania donovani infantum are mixed with a silica sol before gelation occurs. They remain trapped within the growing oxide network and their cellular organization appears to be well preserved. Moreover protozoa retain their antigenic properties in the porous gel. They are still able to detect parasite specific antibodies in serum samples from infected patients via an enzyme linked immunosorbent assay (ELISA). Antigen- antibody associations occurring in the gel are optically detected via the reactions of a peroxidase conjugate with ortho-phenylenediamine leading to the formation of a yellow coloration. A clear-cut difference in optical density is measured between positive and negative sera. Such an entrapment of antigenic species into porous sol-gel matrices avoids the main problems due to non specific binding and could be advantageously used in diagnostic kits.

Citation Download Citation

Jacques Livage, J. Y. Barreau, J. M. Da Costa, and I. Desportes "Optical detection of parasitic protozoa in sol-gel matrices", Proc. SPIE 2288, Sol-Gel Optics III, (13 October 1994); https://doi.org/10.1117/12.188984

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