Spectrofluorimetric analysis in vivo: applications to kinetics of ALA-induced fluorescence

Veronique Vonarx-Coinsmann; S. Cordel; P. Lenz; Marie-Therese Foultier; Roy H. Pottier; James C. Kennedy; Thierry Patrice

doi:10.1117/12.166816

15 January 1994 Spectrofluorimetric analysis in vivo: applications to kinetics of ALA-induced fluorescence

Veronique Vonarx-Coinsmann, S. Cordel, P. Lenz, Marie-Therese Foultier, Roy H. Pottier, James C. Kennedy, Thierry Patrice

Author Affiliations +

Proceedings Volume 2081, Optical Biopsy; (1994) https://doi.org/10.1117/12.166816
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

We designed a laser spectrofluorimeter to determine the optimal delay between drug injection and laser irradiation in which both excitation and fluorescence emission are transmitted through a single optic fiber. Preclinical studies were performed in nude mice after ALA administration. The maximum fluorescence (632 nm) in HT 29 tumors was obtained after 90 min (excitation 488 nm, 20 mW, integration time 3 sec, ALA 250 mg/kg), after 180 min in liver. Iron chelators as EDTA (10 or 20 mg/kg) or desferrioxamine (20 or 40 mg/kg) increased the delay for reaching maximum fluorescence to 2 hours as well as fluorescence intensity in tumors but decreased intensity in liver. Desferrioxamine induced the appearance of protoporphyrin IX in the bladder or kidneys, normally absent in urines. These results indicate that drug distribution varies with tissues and also adjuvant therapies that are often administered together with cancer drugs.

Citation Download Citation

Veronique Vonarx-Coinsmann, S. Cordel, P. Lenz, Marie-Therese Foultier, Roy H. Pottier, James C. Kennedy, and Thierry Patrice "Spectrofluorimetric analysis in vivo: applications to kinetics of ALA-induced fluorescence", Proc. SPIE 2081, Optical Biopsy, (15 January 1994); https://doi.org/10.1117/12.166816

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available