Optogenetic control of arterial diameter in three-dimensions in vivo

Philip J. O'Herron; David A. Hartmann; Kun Xie; Prakash Kara; Andy Y. Shih

doi:10.1117/12.3006878

12 March 2024 Optogenetic control of arterial diameter in three-dimensions in vivo

Philip J. O'Herron, David A. Hartmann, Kun Xie, Prakash Kara, Andy Y. Shih

Proceedings Volume 12829, Optogenetics and Optical Manipulation 2024; 128290G (2024) https://doi.org/10.1117/12.3006878
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Modulation of brain arteriole diameter is critical for maintaining cerebral blood pressure and controlling regional hyperemia during neural activity. However, studies of hemodynamic function in health and disease have lacked a method to control arteriole diameter independently with high spatiotemporal resolution. Here, we describe an all-optical approach to manipulate and monitor brain arteriole contractility in 3-dimensions using combined in vivo two-photon optogenetics and imaging. The expression of the red-shifted excitatory opsin, ReaChR, in vascular smooth muscle cells enabled rapid and repeated vasoconstriction controlled by brief light pulses. Two-photon activation of ReaChR using a spatial light modulator produced highly localized constrictions when targeted to individual arterioles within the neocortex. Wide-field photoactivation with an LED constricted all arteries in the imaging field leading to widespread reductions in blood flow. We demonstrate the utility of this method for examining arteriole contractile dynamics and creating transient focal blood flow reductions. Additionally, we show that optogenetic constriction can be used to reshape vasodilatory responses to sensory stimulation, providing a valuable tool to dissociate blood flow changes from neural activity. We also demonstrate the confound in fluorescence imaging caused by changes in vessel diameter and discuss potential remedies.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Philip J. O'Herron, David A. Hartmann, Kun Xie, Prakash Kara, and Andy Y. Shih "Optogenetic control of arterial diameter in three-dimensions in vivo", Proc. SPIE 12829, Optogenetics and Optical Manipulation 2024, 128290G (12 March 2024); https://doi.org/10.1117/12.3006878

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