Registration of hyperspectral images and mass spectrometry data for the correlation of tissue optical spectra and molecular profiles

Angie T. Fei; Douglas W. Strand; Jing Wang

doi:10.1117/12.3007718

12 March 2024 Registration of hyperspectral images and mass spectrometry data for the correlation of tissue optical spectra and molecular profiles

Angie T. Fei, Douglas W. Strand, Jing Wang

Author Affiliations +

Proceedings Volume 12827, Multiscale Imaging and Spectroscopy V; 1282708 (2024) https://doi.org/10.1117/12.3007718
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Hyperspectral imaging (HSI) is a label-free imaging modality that is emerging for non-invasive detection of various diseases including cancers. HSI provides high-resolution spatial images where each pixel has a spectral curve with numerous wavelength bands from the visible to infrared ranges. The rich spatial and spectral information can be used to discriminate various types of tissues and pathophysiological conditions. However, it can be difficult to explain spectral data with respect to the underline cellular and molecular mechanism. In this study, we developed an approach that registers hyperspectral images and mass spectrometry (MS) data where MS provides tissue molecular profiles. Human prostate tissues that were obtained after prostatectomy were used in the experiments. The whole prostate was first sliced every six mm. A customized hyperspectral surgical microscope was used to acquire HSI data from the sliced tissue. For MS data analysis, the sliced tissue of the prostate was divided into 51 small regions and then processed separately for each region. The immediately adjacent tissue was sliced and processed histologically for H&E staining. The MS molecular profiles were correlated with the hyperspectral images in this study.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Angie T. Fei, Douglas W. Strand, and Jing Wang "Registration of hyperspectral images and mass spectrometry data for the correlation of tissue optical spectra and molecular profiles", Proc. SPIE 12827, Multiscale Imaging and Spectroscopy V, 1282708 (12 March 2024); https://doi.org/10.1117/12.3007718

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