Metabolic perturbations of macrophages on engineered collagen scaffolds with label-free fluorescence lifetime imaging (Conference Presentation)

Linghao Hu; Dhavan Sharma; Feng Zhao; Alex J. Walsh

doi:10.1117/12.2650468

14 March 2023 Metabolic perturbations of macrophages on engineered collagen scaffolds with label-free fluorescence lifetime imaging (Conference Presentation)

Linghao Hu, Dhavan Sharma, Feng Zhao, Alex J. Walsh

Author Affiliations +

Proceedings Volume 12380, Biophotonics and Immune Responses XVIII; 123800G (2023) https://doi.org/10.1117/12.2650468
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

In this study, we engineered a vascularized 3D dermis by layering multiple highly aligned prevascularized sheets with human mesenchymal stem cells to mimic the capillary loops observed in native dermis tissue. Monocyte-derived macrophages were seeded onto these prevascularized constructs to mimic the innate immune-response in vitro. To evaluate macrophage responses to these scaffolds, we used multiphoton microscopy to detect the metabolic phenotypes of macrophages on engineered scaffolds over time. NADH and FAD are coenzymes that emit endogenous fluorescence and participate in various metabolic pathways, and their fluorescence lifetime is associated with different protein binding states and microenvironments. Fluorescence lifetime images of macrophages were obtained on day 3 and day 7 after seeding, and significant differences in NADH and FAD fluorescence lifetime features can be observed in macrophages on different scaffolds.

Conference Presentation

Citation Download Citation

Linghao Hu, Dhavan Sharma, Feng Zhao, and Alex J. Walsh "Metabolic perturbations of macrophages on engineered collagen scaffolds with label-free fluorescence lifetime imaging (Conference Presentation)", Proc. SPIE 12380, Biophotonics and Immune Responses XVIII, 123800G (14 March 2023); https://doi.org/10.1117/12.2650468

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