High-resolution human brain 3D reconstruction with light-sheet fluorescence microscopy

Irene Costantini; Marina Scardigli; Josephine Ramazzotti; Niamh Brady; Franco Cheli; Giacomo Mazzamuto; Filippo Castelli; Curzio Checcucci; Ludovico Silvestri; Paolo Frasconi; Francesco Saverio Pavone

doi:10.1117/12.2649323

14 March 2023 High-resolution human brain 3D reconstruction with light-sheet fluorescence microscopy

Irene Costantini, Marina Scardigli, Josephine Ramazzotti, Niamh Brady, Franco Cheli, Giacomo Mazzamuto, Filippo Castelli, Curzio Checcucci, Ludovico Silvestri, Paolo Frasconi, Francesco Saverio Pavone

Author Affiliations +

Proceedings Volume 12365, Neural Imaging and Sensing 2023; 1236503 (2023) https://doi.org/10.1117/12.2649323
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

3D reconstruction of the human brain at high resolution is one of the most important challenges of neuroscience. We present a new clearing method named SHORT that in combination with an advanced double-view light-sheet fluorescence microscope and an automated machine-learning based images analysis allow to perform volumetric study of the human brain. We applied our methodology to a Broca’s area block of 4 x 4 x 2 cm³, demonstrating the possibility of obtaining a fast 3D reconstruction of the human brain at high-resolution, paving the way to the possibility of finally mapping a comprehensive atlas of the human brain.

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Irene Costantini, Marina Scardigli, Josephine Ramazzotti, Niamh Brady, Franco Cheli, Giacomo Mazzamuto, Filippo Castelli, Curzio Checcucci, Ludovico Silvestri, Paolo Frasconi, and Francesco Saverio Pavone "High-resolution human brain 3D reconstruction with light-sheet fluorescence microscopy", Proc. SPIE 12365, Neural Imaging and Sensing 2023, 1236503 (14 March 2023); https://doi.org/10.1117/12.2649323

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