Assessing membrane charging by alternating electric fields in giant unilamellar vesicles

Allen Kiester; Stacey L. Martens; Zachary Coker; Mara Casebeer; Joel Bixler; Esin B. Sözer; Bennett L. Ibey

doi:10.1117/12.2578299

5 March 2021 Assessing membrane charging by alternating electric fields in giant unilamellar vesicles

Allen Kiester, Stacey L. Martens, Zachary Coker, Mara Casebeer, Joel Bixler, Esin B. Sözer, Bennett L. Ibey

Proceedings Volume 11640, Optical Interactions with Tissue and Cells XXXII; 1164009 (2021) https://doi.org/10.1117/12.2578299
Event: SPIE BiOS, 2021, Online Only

Abstract

Our group recently published direct observation of membrane charging in FluoVolt labeled CHO-K1 cells by nanosecond electrical pulses using a streak camera. Using this technique, called Streak Camera Microscopy (SCM), we imaged the membrane charging dynamics in giant unilamellar vesicles (GUVs) during AC exposures up to 6 MHz and compared these results to existing capacitive circuit models of membranes. This work shows further application of Streak Camera Microscopy for evaluation of high speed biological events.

Conference Presentation

Citation Download Citation

Allen Kiester, Stacey L. Martens, Zachary Coker, Mara Casebeer, Joel Bixler, Esin B. Sözer, and Bennett L. Ibey "Assessing membrane charging by alternating electric fields in giant unilamellar vesicles", Proc. SPIE 11640, Optical Interactions with Tissue and Cells XXXII, 1164009 (5 March 2021); https://doi.org/10.1117/12.2578299

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Microscopy

Particle filters

Streak cameras

Cameras

Luminescence

Neurophotonics

Optogenetics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years