1700 nm optical coherence microscopy enables minimally invasive, volumetric, deep tissue optical biopsy of the mouse brain in vivo

Jun Zhu; Hercules Freitas; Izumi Maezawa; Lee-Way Jin; Vivek J. Srinivasan

doi:10.1117/12.2577001

5 March 2021 1700 nm optical coherence microscopy enables minimally invasive, volumetric, deep tissue optical biopsy of the mouse brain in vivo

Jun Zhu, Hercules Freitas, Izumi Maezawa, Lee-Way Jin, Vivek J. Srinivasan

Author Affiliations +

Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 1162910 (2021) https://doi.org/10.1117/12.2577001
Event: SPIE BiOS, 2021, Online Only

Abstract

Neuroimaging techniques aim to image deep, with high resolution and minimal invasiveness. Here, we present a label-free optical microscopy approach that achieves a unique balance between these competing goals. Specifically, we design a high numerical aperture optical coherence microscope centered near 1700 nm, where ballistic attenuation in the mouse brain is minimized. Dynamic focusing and image fusion are employed to balance speckle reduction against multiple scattered light reduction. Imaging through the thinned skull to preserve intracranial pressure and minimize inflammation, we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire mouse neocortex and some sub-cortical regions.

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Jun Zhu, Hercules Freitas, Izumi Maezawa, Lee-Way Jin, and Vivek J. Srinivasan "1700 nm optical coherence microscopy enables minimally invasive, volumetric, deep tissue optical biopsy of the mouse brain in vivo", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 1162910 (5 March 2021); https://doi.org/10.1117/12.2577001

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KEYWORDS

Tissue optics

Brain

In vivo imaging

Biopsy

Optical coherence microscopy

Coherence (optics)

Neuroimaging

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