Correlation analysis of human iPSC-derived neuronal networks using holographic single cell and full field stimulation

Felix Schmieder; Rouhollah Habibey; Volker Busskamp; Lars Büttner; Jürgen W. Czarske

doi:10.1117/12.2583240

5 March 2021 Correlation analysis of human iPSC-derived neuronal networks using holographic single cell and full field stimulation

Felix Schmieder, Rouhollah Habibey, Volker Busskamp, Lars Büttner, Jürgen W. Czarske

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Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 116290M (2021) https://doi.org/10.1117/12.2583240
Event: SPIE BiOS, 2021, Online Only

Abstract

Optogenetics combined with microelectrode array electrophysiology is a valuable tool set for the investigation of structure-function relations e.g. for understanding of human neurodegenerative diseases at microscale levels. We conducted long-term electrophysiology, full field and holographic single-neuron stimulation experiments using a fast ferroelectric modulator on in-vitro networks of iNGN cells derived from human fibroblasts expressing wild-type Channelrhodopsin2-EYFP. Functional maturation and successful ChR2-expression are observed in significant rises in action potential frequencies. Single neuron stimulation allows the extraction of functional connectivity maps, signal origins and post- or presynaptic signal characteristics as well as other modalities we will discuss in our presentation.

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Felix Schmieder, Rouhollah Habibey, Volker Busskamp, Lars Büttner, and Jürgen W. Czarske "Correlation analysis of human iPSC-derived neuronal networks using holographic single cell and full field stimulation", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116290M (5 March 2021); https://doi.org/10.1117/12.2583240

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KEYWORDS

Holography

Electrodes

Brain mapping

Electrophysiology

In vitro testing

Network security

Optogenetics

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